Gram stain of tissue 活组织检查

定义

Gram stain of tissue 活组织检查 test involves using crystal violet stain to test a sample of tissue taken from a 活组织检查.

The Gram stain method can be used on almost any specimen. It is an excellent technique for making a general, basic identification of the type of bacteria in the sample.

选择的名字

Tissue 活组织检查 - Gram stain

How the Test is Performed

一个示例, 叫做涂片, from a tissue specimen is placed in a very thin layer on a microscope slide. The specimen is stained with crystal violet stain and goes through more processing before it is examined under the microscope for bacteria.

The characteristic appearance of the bacteria, such as their color, 形状, clustering (if any), and pattern of staining help determine the type of bacteria.

How to Prepare for the Test

If the 活组织检查 is included as part of a surgical procedure, you will be asked not to eat or drink anything the night before surgery. If the 活组织检查 is of a superficial (on the surface of the body) tissue, you may be asked not to eat or drink for several hours before the procedure.

How the Test will Feel

How the test feels depends on the part of the body being biopsied. There are several different methods for taking tissue samples.

• A needle may be inserted through the skin to the tissue.
• A cut (incision) through the skin into the tissue may be made, and a small piece of the tissue removed.
• A 活组织检查 may also be taken from inside the body using an instrument that helps the doctor see inside the body, such as an endoscope or cystoscope.

You may feel pressure and mild pain during a 活组织检查. Some form of pain-relieving medicine (anesthetic) is usually given, so you have little or no pain.

Why the Test is Performed

The test is performed when an infection of a body tissue is suspected.

正常的结果

Whether there are bacteria, and what type there are, depends on the tissue being biopsied. Some tissues in the body are sterile, such as the brain. Other tissues, such as the gut, normally contain bacteria.

Note: Normal value ranges may vary slightly among different laboratories. Talk to your doctor about the meaning of your specific test results.

What Abnormal Results Mean

Abnormal results usually mean there is an infection in the tissue. 更多的测试, such as culturing the tissue that was removed, are often needed to identify the type of bacteria.

风险

风险 depend on the procedure used to take the tissue 活组织检查, and may include bleeding or infection.

参考文献

Chernecky CC, Berger BJ. Biopsy, site-specific - specimen. In: Chernecky CC, Berger BJ, eds. Laboratory Tests and Diagnostic Procedures. 6日艾德. St Louis, MO: Elsevier Saunders; 2013:199-202.

Wojewoda CM, Stempak LM. Medical bacteriology. In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 24日艾德. Philadelphia, PA: Elsevier; 2022:chap 57.